Sodium Hydroxide → HCID 71205653, compound

Participants

Reaction Steps

Intermediate 69 (147 mg) was dissolved in Methanol (4 mL). NaOH (2 mL) was added to the stirred reaction mixture as a 3.3 N aqueous soln and the reaction mixture stirred at room temperature. After stirring overnight, LCMS of the reaction mixture demonstrated full consumption of the starting material and the formation of the desired acid product. The reaction mixture was conc in vacuo and taken up in acetonitrile/water. The soln was taken to pH4-5 by the addition of 2 M aqueous HCl and loaded onto an SCX column. After washing with water and acetonitrile, the product was eluted with 2 M ammonia soln in methanol. The washings were conc in vacuo to afford the ammonium salt of the desired product: LC/MS MH+ 361, Rt 2.02 mins (5 min run).

Provenance & Evidence