反应详情

Ethyl 3-amino-6-(1,4-dioxaspiro[4.5]dec-8-yl)pyridine-2-carboxylate (Compound 232E, 228 mg, 0.744 mmol) was taken up in THF (1.0 mL) and MeOH (1.0 mL, 25 mmol). This was treated with a solution of Lithium hydroxide, monohydrate (156 mg, 3.72 mmol) in H2O (1.0 mL) and allowed to stir at rt for 4 hours. The THF was removed in vacuo and treated, dropwise with 1N HCl until a pH of ˜5 was achieved. This aqueous solution was extracted with EtOAc (3×50 mL) and saturated with salt and stirred with ˜100 mL DCM. The combined organic layers were dried over Na2SO4, filtered and concentrated to a crude yellow oil which was used without further purification. MS (ES+): m/z=278.86 [MH+]. HPLC: tR=2.39 min (ZQ3, polar—5 min).

1. customThe THF was removed in vacuo
2. additiontreated, dropwise with 1N HCl until a pH of ˜5
3. extractionThis aqueous solution was extracted with EtOAc (3×50 mL) and saturated with salt
4. stirringstirred with ˜100 mL DCM
5. dry with materialThe combined organic layers were dried over Na2SO4
6. filtrationfiltered
7. concentrationconcentrated to a crude yellow oil which
8. customwas used without further purification
9. custom5 min