HuagongsheHuagongshe Chemistry Print Report · For research reference only
Acetate → Retinoic Acid
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Acetate | HCID 175 | — |
| Reactant | Cholesterol | HCID 5997 | — |
| Reactant | Dimethylformamide | HCID 6228 | — |
| Solvent | Methanol | HCID 887 | — |
| Product | Retinoic Acid | HCID 444795 | — |
Reaction Steps
40 mg of hapten are solubilized in 2 ml of anhydrous methanol and 2 ml of anhydrous DMF containing 1 μl of radioactive tracer (cholesterol: ref. NET 725, Dupont de Nemours). 80 mg of PL dissolved in 2 ml of 1.5 M acetate buffer are then added dropwise. After thorough stirring, the conjugates are purified by dialysis. The first dialysis solution consists of a mixture of 1/3 of methanol, 1/3 of DMF and 1/3 of water. The following two solutions consist solely of water.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US06114388 |
| Source dataset | uspto-grants-2000_09 |
| ORD ID | ord-88a5ee229d9443b8b1315e3ebb2e83b8 |
| Source note | uspto-grants-2000_09 |