3-Hydroxyleucine → L-Leucine

HRID 1954660Notes

Reaction equation

HRID 1954660 reaction equation
O[CH:2]([CH:8]([CH3:10])[CH3:9])[CH:3]([C:5]([OH:7])=[O:6])[NH2:4].ON[C@H](C(O)=O)CC(C)C>>[NH2:4][C@H:3]([C:5]([OH:7])=[O:6])[CH2:2][CH:8]([CH3:10])[CH3:9]

Participants

3

Reactant 2

HCID 2777763-HydroxyleucineHCID 20759852HCID 20759852, compound2×

Product 1

HCID 6106L-Leucine

Procedure

A mutant strain which was resistant to 5 g/L of L-valine was selected from E. coli K-12 and obtained the strain No. 101 (Valr), which did not produce L-leucine. From the strain No. 101, a mutant strain which was resistant to 1.3 g/L of azaleucine. The obtained strain, No. 51 (Varr, ALr), produced about 0.05-0.1 g/L of L-leucine. Then, a strain having a resistance to 2 g/L of 3-hydroxy-D,L-leucine (hereinafter referred to as hydroxyleucine) was selected from the strain No. 51 and obtained the strain No.4 (Varr, ALr, Hleur). The symbol, "Hleur " represents the strain which was conferred hydroxyleucine resistance. The strain No. 4 produced more leucine (about 0.4-0.6 g/L).

Workup

  1. custom51 and obtained the strain No.4 (Varr, ALr, Hleur)

Sources & evidence

ORD record
ord-52c69a02c9994ad48ee5b78e19275a1c
Source dataset
uspto-grants-2000_09
Patent number
US06124121

Reaction notes

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