Phosphoric Acid → Paclitaxel

Participants

Reaction Steps

HPLC analysis for paclitaxel content in liposome formulations was performed on a 15 cm long pentafluorophenyl (PFP) column (ES Industries, Inc.) with a Waters 501 HPLC pump and a Waters 991 photodiode array detector. The flow rate and temperature were controlled at 1.5 ml/min. and 27° C., respectively. The mobile phase consisted of 44% acetonitrile, 56% water, and about 0.1% (vol.) of phosphoric acid. This system gave retention times for paclitaxel and cephalomannine around 12.5 and 9.5 minutes, respectively, with UV detection at 228 nm. The HPLC chromatogram of the paclitaxel/cephalomannine (taxoid) mixture is presented in FIG. 7.

Provenance & Evidence