Phenazine Methosulfate → 2-keto-L-Gulonic acid

Participants

Reaction Steps

A reaction mixture containing the purified aldehyde dehydrogenase (0.04 mg protein, and prepared according to Example 1), L-sorbosone (4 mg) and phenazine methosulfate (0.2 mg) in 1.0 ml of 0.5M potassium phosphate buffer (pH 7.0) was incubated for 1.0 hour at 25° C. with gentle shaking. As a result, 2-keto-L-gulonic acid was formed at the rate of about 2.3 mg/hour.

Provenance & Evidence