HuagongsheHuagongshe Chemistry Print Report · For research reference only
Hydrochloric Acid → Phosphate Buffered Saline
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Hydrochloric Acid | HCID 313 | — |
| Reactant | Potassium Chloride | HCID 4873 | — |
| Reactant | Sodium Chloride | HCID 5234 | — |
| Reactant | Sodium Phosphate, Dibasic | HCID 24203 | — |
| Reactant | Sodium Azide | HCID 33557 | — |
| Reactant | Monobasic potassium phosphate | HCID 516951 | — |
| Solvent | Water | HCID 962 | — |
| Product | Phosphate Buffered Saline | HCID 24978514 | — |
Reaction Steps
Dulbecco's phosphate buffered saline (DPBS) is prepared by first dissolving, sequentially, about 160 g sodium chloride (NaCl), about 4 grams potassium chloride (KCl), about 23 grams disodium hydrogen orthophosphate (Na2HPO4), about 4 grams potassium dihydrogen orthophosphate (KH2PO4), and about 10 grams sodium azide in a liter of reverse-osmosis (MilliQ) water. Then, the pH is adjusted to about 7.3 by adding appropriate amounts of HCl. Finally, the buffer solution is diluted to 1:20 (50 ml buffer solution with 950 ml reverse-osmosis water), and allowed to degas either in a screw-capped container overnight or under vacuum.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US05776999 |
| Source dataset | uspto-grants-1998_07 |
| ORD ID | ord-45798841ae004841a635d740e2739d56 |
| Source note | uspto-grants-1998_07 |