反应详情

A solution of the compound of step 5 (158 mg, 299 mmol) in DCM (8 ml) and TFA (4 ml) was stirred at room temperature for 2 h. The solvents were evaporated and the residue was purified by preparative HPLC. The fractions containing the title compound were combined and lyophilized overnight. The obtained solid was dissolved in a small quantity of MOH, mixed with 0.1 N hydrochloric acid and the mixture lyophilized overnight to give 108 mg of the title compound in the form of [2-(3-fluoro-2-methyl-benzyl)-3-phenyl-indolizin-1-yl]-piperazin-1-yl-methanone hydrochloride with the following characteristics:

1. customThe solvents were evaporated
2. customthe residue was purified by preparative HPLC
3. additionThe fractions containing the title compound
4. dissolutionThe obtained solid was dissolved in a small quantity of MOH
5. additionmixed with 0.1 N hydrochloric acid
6. waitthe mixture lyophilized overnight