反应详情

{5-(Bromomethyl)-3-[4-(cyclohexylamino)-1-ethyl-1H-pyrazolo[3,4-b]pyridin-5-yl]-4,5-dihydroisoxazol-5-yl}methanol (110 mg, 0.00025 mole) (example 20) was dissolved in ethanol (10 ml). Water (2 ml) was added followed by potassium hydroxide (20 mg, 0.00050 mole). The reaction mixture was stirred at refluxing temperature overnight. The solvent was removed under reduced pressure. Water was added and the mixture was extracted with ethyl acetate, washed with brine, dried over anhydrous sodium sulphate and concentrated in vacuo. The crude product obtained was purified by column chromatography.

1. temperatureat refluxing temperature overnight
2. customThe solvent was removed under reduced pressure
3. additionWater was added
4. extractionthe mixture was extracted with ethyl acetate
5. washwashed with brine
6. dry with materialdried over anhydrous sodium sulphate
7. concentrationconcentrated in vacuo