反应详情

((1S,2S)-1-{[[(1S,2S)-2-(6-Fluoro-pyridin-2-yl)-cyclopropanecarbonyl]-(4-pent-1-ynyl-phenyl)-amino]-methyl}-2-methyl-butyl)-carbamic acid t-butyl ester (0.0657 g, 0.126 mmol), trifluoroacetic acid (1 mL) and dichloromethane (2 mL) were mixed at 0° C., then stirred at room temperature for 1 hr. The excessive solvent was removed in vacuum. The residue was purified by preparatory HPLC to give a white solid (0.0277 g, 34%): 1H NMR (400 MHz, MeOD) δ ppm 7.66 (m, 1H), 7.24 (m, 4H), 7.07 (td, J=7.1, 2.3 Hz, 1H), 6.66 (m, 1H), 4.16 (m, 1H), 3.58 (m, 1H), 3.21 (dt, J=3.3, 1.6 Hz, 1H), 2.47 (br. s., 1H), 2.29 (td, J=7.0, 3.2 Hz, 2H), 1.81 (dd, J=5.3, 3.5 Hz, 1H), 1.62 (m, 1H), 1.53 (m, 3H), 1.29 (m, 2H), 1.10 (m, 1H), 0.95 (td, J=7.3, 2.5 Hz, 3H), 0.86 (dd, J=7.1, 4.8 Hz, 3H), 0.73 (m, 3H); LCMS (ESI) m/e 422.3 [(M+H)+, calcd for C26H33FN3O 422.6].

1. customThe excessive solvent was removed in vacuum
2. customThe residue was purified by preparatory HPLC