反应详情

To a solution of 65 mg 4-[(S)-2-({5-[2-((S)-2-Cyclobutylcarbamoyl-pyrrolidin-1-yl)-2-oxo-ethoxy]-1-phenyl-1H-pyrazole-3-carbonyl}-amino)-5-hydroxy-pentanoyl]-piperazine-1-carboxylic acid butyl ester in 4 ml dichloromethane were added 15 l pyridine, 10 l acetic anhydride and a catalytic amount of DMAP. After stirring for 24 h at room temperature the reaction mixture was concentrated and the crude product thus obtained was purified by preparative HPLC (C18 reverse phase column, elution with a water/MeCN gradient with 0.1% TFA) to give the title compound.

1. concentrationwas concentrated
2. customthe crude product thus obtained
3. customwas purified by preparative HPLC (
4. washC18 reverse phase column, elution with a water/MeCN gradient with 0.1% TFA)