Cesium carbonate → HCID 86670329, compound

Participants

Reaction Conditions

Reaction Steps

2-(3-bromo-4-(4-(2-thiophenylsulfonyl)-1-piperazinyl)phenyl)-1,1,1,3,3,3-hexafluoro-2-propanol (90 mg, 0.163 mmol), phenylboronic acid (23.8 mg, 0.195 mmol, Sigma-Aldrich, St. Louis, Mo.), 1,1′ bis(diphenylphosphino)ferrocene]dichloropalladium (II) (13.28 mg, 0.016 mmol, Sigma-Aldrich, St. Louis, Mo.), and cesium carbonate (159 mg, 0.488 mmol) were combined in THF (3 mL) and water (1 mL). The reaction mixture was stirred and heated in a Discover model microwave reactor (CEM, Matthews, N.C.) at 100° C. for 10 min (100 watts, Powermax feature on, ramp time 5 min). The organic layer was separated and filtered through a PTFE (polytetrafluoroethylene) filter (0.45 micron). This crude material was subjected to reverse-phase preparative HPLC using a Phenomenex Gemini C18 column (150×30 mm, 10 μm) eluting with 0.1% TFA in CH3CN/H2O (10% to 90% over 20 min) to provide 1,1,1,3,3,3-hexafluoro-2-(6-(4-(2-thiophenylsulfonyl)-1-piperazinyl)-3-biphenylyl)-2-propanol trifluoroacetate (43 mg) as a tan oil. 1H NMR (300 MHz, CD3OD) δ 7.89 (d, J=4.8 Hz, 1H), 7.68-7.39 (m, 5H), 7.38-7.20 (m, 4H), 7.14 (d, J=8.6 Hz, 1H), 2.93 (br. s, 8H). m/z (ESI, +ve ion) 550.8 (M+H)+. GK-GKRP EC50 (NADPH-coupled)=2.12 μM; GK-GKRP EC50 (LC MS/MS)=1.75 μM.

Provenance & Evidence