Ammonium Chloride → HCID 66582477, compound

Participants

Reaction Steps

A solution of 1-((2S)-4-(2-thiophenylsulfonyl)-1-(4-(2,2,2-trifluoro-1-hydroxy-1-methylethyl)phenyl)-2-piperazinyl)-2-propanone (100 mg, 0.210 mmol) and oxetan-3-amine (18.41 mg, 0.252 mmol, J & W PharmLab, Levittown, Pa.) in THF (10 mL) was cooled to 0° C. To this mixture was added Ti(O-iPr)4 (154 μL, 0.525 mmol, Sigma-Aldrich, St. Louis, Mo.). The mixture was stirred for 3 h and then a solution of sodium borohydride (11.91 mg, 0.315 mmol, Sigma-Aldrich, St. Louis, Mo.) in MeOH (1 mL) was added. The reaction mixture was stirred at ambient temperature for 4 h. Saturated aqueous NH4Cl was added and the mixture was filtered. The filtrate was concentrated and purified via reverse-phase preparative HPLC using a Phenomenex Gemini C18 column (150×30 mm, 10 μm) eluting with 0.1% TFA in MeCN/H2O (5% to 100% over 15 min) to give 1,1,1-trifluoro-2-(4-((2S)-2-(2-(3-oxetanylamino)propyl)-4-(2-thiophenylsulfonyl)-1-piperazinyl)phenyl)-2-propanol (28 mg) as a mixture of four isomers

Provenance & Evidence