Sodium periodate → HCID 66583085, compound

Participants

Reaction Steps

A solution of (2S)-1,1,1-trifluoro-2-(4-(2-(((R)-3-methylmorpholino)methyl)-4-(thiophen-2-ylsulfonyl)piperazin-1-yl)phenyl)propan-2-ol (50 mg, 0.094 mmol, Example 45, peak 2) in EtOAc (1 mL) was added to a mixture of dioxoruthenium hydrate (1.42 mg, 9.37 μmol, Sigma Aldrich, St. Louis, Mo.) and 10% aqueous sodium periodate (30.1 mg, 0.141 mmol, Sigma Aldrich, St. Louis, Mo.) in water (1.5 mL). The solution was stirred vigorously for 48 hours at room temperature. The reaction mixture was then concentrated in a GeneVac HT-12 (GeneVac Technologies, IPS Technologies, UK), re-dissolved in MeOH (1.5 mL), filtered through syringe filters (Pall Life Sciences, Acrodic®, 25 mm Syringe Filter w/0.45 μm HT Tuffryn® Membrane) and purified on Prep HPLC (Method: 10 min, 10 to 90% ACN, Water, 0.1% TFA, 254 nm; Column: Phenomenex, Gemini-NX, 5μ, C18, 150×30.00 mm) to afford (S)-5-methyl-4-(((S)-4-(thiophen-2-ylsulfonyl)-1-(4-((S)-1,1,1-trifluoro-2-hydroxypropan-2-yl)phenyl)piperazin-2-yl)methyl)morpholin-3-one (12.6 mg).

Provenance & Evidence