反应详情

5'-azido-2',5'-dideoxyuridine (258 mg, 1 mmole) is dissolved in 50 ml of ethanol and 50 mg of 10% palladium carbon catalyst added. The suspension is maintained at atmospheric pressure in a hydrogenation apparatus for 2.5 hours before filtering through Celite. The solvent is removed in vacuo, the residue dissolved in water and applied to a column of Dowex 50 (H+) resin. After washing the column with 100 ml of water, the 5'-amino-2',5'-dideoxyuridine is eluted with 100 ml of 1M NH4OH. The solution is evaporated to dryness and the residue crystallized from ethanol-ether to provide the desired product, (127 mg, 56%).

1. temperatureThe suspension is maintained at atmospheric pressure in a hydrogenation apparatus for 2.5 hours
2. filtrationbefore filtering through Celite
3. customThe solvent is removed in vacuo
4. dissolutionthe residue dissolved in water
5. washAfter washing the column with 100 ml of water
6. washthe 5'-amino-2',5'-dideoxyuridine is eluted with 100 ml of 1M NH4OH
7. customThe solution is evaporated to dryness
8. customthe residue crystallized from ethanol-ether