Phosphate → 2-Nitrophenol

HRID 1974441Notes

Reaction equation

HRID 1974441 reaction equation
P([O-])([O-])([O-])=O.[CH:6]1[CH:11]=[C:10]([N+:12]([O-:14])=[O:13])[C:9]([O:15]C2OC(CO)C(O)C(O)C2O)=[CH:8][CH:7]=1.C([O-])([O-])=O.[Na+].[Na+]>>[N+:12]([C:10]1[CH:11]=[CH:6][CH:7]=[CH:8][C:9]=1[OH:15])([O-:14])=[O:13]

Participants

4

Reactant 3

HCID 1061PhosphateHCID 10340Sodium CarbonateHCID 2199732816-24-22×

Product 1

HCID 69472-Nitrophenol

Procedure

That is, 0.1 ml of the present enzyme solution is added to 2.4 ml of 0.1M phosphate buffer (pH 6.5) containing 1.50% o-nitrophenyl-β-D-galactopyranoside (hereinafter referred to as "ONPG") and reacted at 70° C. for 10 minutes. Thereafter, the reaction is discontinued by the addition of 2.5 ml of 10% Na2CO3 solution. The amount of o-nitrophenol formed is obtained from the absorbance at 420 nm, and the amount of enzyme liberating 1 μmol of o-nitrophenol per minute is taken as one unit.

Workup

  1. customreacted at 70° C. for 10 minutes

Sources & evidence

ORD record
ord-6513a5dbb8094ae39886407eb9c53ffa
Source dataset
uspto-grants-1994_02
Patent number
US05283189

Reaction notes

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