反应详情

温度

32 °C

One ml of the reaction solution containing 100 μmol of potassium phosphate buffer solution (pH 8.0), 4.77 μmol of 2-(4'-isobutylphenyl)propionitrile and 0.02 unit of nitrilase was thoroughly shaken at 32° C. to carry out the reaction. The amounts of 2-(4'-isobutylphenyl)-propionitrile, 2-(4'-isobutylphenyl)propionic acid and ammonia for each reaction time were determined. The results are shown in the Figure. The optical purity of the formed S-(+)-2-(4'-isobutylphenyl)propionic acid was 98% for 6 hours, 96% for 24 hours and 95% for 40 hours.

1. customthe reaction
2. customfor 6 hours
3. wait95% for 40 hours