反应详情

温度

28 °C

Strain K1/1 is grown according to example 3 in a 30 l fermenter filled with 20 l of basic medium containing 1 g/l of racemic N-acetyl-1-phenylethylamine. The cells are harvested by continuous centrifugation and resuspended in the reaction mixture, which is prepared by adding a solution of 80 g racemic 2-acetylamino-1(4-chlorophenyl)-4-pentene in 500 ml methanol to 20 l of potassium phosphate buffer, 69 mM, pH 7. After stirring for 21 h at 28° C. and 500 rpm the amine formed and the unreacted amide are separated and analyzed for their optical purity analogously to the procedure given in example 30.

1. additionfermenter filled with 20 l of basic medium
2. customis prepared