N-Hydroxysuccinimide → HCID 21994017, compound

Participants

Reaction Steps

To a suspension of 1.0 g (4.58 mM) of 5-thia-1,8b-diazaacenaphthylene-4-carboxylic acid and 0.90 g (7.82 mM) of N-hydroxysuccinimide in acetonitrile (10 ml) was added 1.51 g (7.88 mM) of N-ethyl-N′-3-(N,N-dimethylamino)propylcarbodiimide hydrochloride at room temperature and the mixture was stirred at the prevailing temperature for 2 hours. To this reaction mixture was added a solution of 2.02 g (5.89 mM) of 1-(4-aminobutan-1-yl)-4-phenylpiperazine trihydrochloride and 4.1 ml (29.4 mM) of triethylamine in ethanol (10 ml) and the mixture was further stirred for one hour. The solvent was distilled off under reduced pressure and the residue was diluted with water and extracted with chloroform. The organic layer was washed with saturated aqueous solution of sodium chloride, dried over MgSO4, and purified by column chromatography (methanol/ethyl acetate 50% methanol-chloroform=1:10). After concentration, ethanol was added to the residue and the resulting crystal crop was harvested by filtration to provide the title compound as rouge-colored crystals.

Provenance & Evidence