反应详情

温度

22 °C

1-Hydroxybenzotriazole (3.24 g) and N,N'-dicyclohexylcarbodiimide (4.95 g) are added to a solution, cooled to +5° C., of 7-amino-2-benzhydryloxycarbonyl-8-oxo-3-[2-(pyridin-3-yl-amino)-thiazol-5-yl]-5-thia-1-azabicyclo[ 4.2.0]oct-2-ene (10.83 g) and the syn isomer of 2-(2-tritylaminothiazol-4-yl)-2-[(2-t-butoxycarbonylprop-2-yl)-oxyimino]-acetic acid (13.72 g) in anhydrous N,N-dimethylformamide (200 cc). The mixture is stirred for 30 minutes at 5° C. and for 3 hours 30 minutes at 22° C. Acetic acid (1 cc) is added, the mixture is then filtered, the precipitate is washed with dimethylformamide (20 cc) and the filtrate is diluted with ethyl acetate (900 cc). The organic phase is washed with distilled water (2×500 cc), a 0.1 N aqueous solution of hydrochloric acid (500 cc), a semi-saturated solution of sodium bicarbonate (500 cc), distilled water (2×500 cc) and a saturated solution of sodium chloride (500 cc). It is dried over anhydrous magnesium sulphate; the mixture is filtered and the filtrate is concentrated to dryness under reduced pressure (30 mm Hg; 4 kPa) at 30° C. The residue is purified by chromatography on a column (height=63 cm, diameter=4.8 cm), containing silica gel (0.06-0.20 mm), elution being carried out under a pressure of 0.4 bar with a mixture of cyclohexane and ethyl acetate (50/50 by Volume) (4 liters) and 250 cc fractions being collected. Fractions 8 to 14, containing the pure product, are combined and concentrated under reduced pressure (30 mm Hg: 4 kPa) at 30° C. to give the syn isomer of 2-benzhydryloxycarbonyl-7-{2-[(2-t-butoxycarbonylprop-2-yl)-oxyimino]-2-(2-tritylaminothiazol-4-yl)-acetamido}-8-oxo-3-[2-(pyridin-3-yl-amino)-thiazol-5-yl]-5-thia-1-azabicyclo[4.2.0] oct-2-ene (4.65 g) in the form of a hard yellow foam.

1. filtrationthe mixture is then filtered
2. washthe precipitate is washed with dimethylformamide (20 cc)
3. additionthe filtrate is diluted with ethyl acetate (900 cc)
4. washThe organic phase is washed with distilled water (2×500 cc)
5. distillationa 0.1 N aqueous solution of hydrochloric acid (500 cc), a semi-saturated solution of sodium bicarbonate (500 cc), distilled water (2×500 cc)
6. dry with materialIt is dried over anhydrous magnesium sulphate
7. filtrationthe mixture is filtered
8. concentrationthe filtrate is concentrated to dryness under reduced pressure (30 mm Hg; 4 kPa) at 30° C
9. customThe residue is purified by chromatography on a column (height=63 cm, diameter=4.8 cm)
10. additioncontaining
11. washsilica gel (0.06-0.20 mm), elution
12. additionbeing carried out under a pressure of 0.4 bar with a mixture of cyclohexane and ethyl acetate (50/50 by Volume) (4 liters) and 250 cc fractions
13. custombeing collected
14. additionFractions 8 to 14, containing the pure product
15. concentrationconcentrated under reduced pressure (30 mm Hg: 4 kPa) at 30° C.