Acetic Anhydride → HCID 18603086, compound

Participants

Reaction Conditions

Reaction Steps

An ester analog of PAF, 1-hexadecanoyl-2-acetyl-sn-glycero-3-phosphocholine, was made by acetylation of 1-hexadecanoyl-2-lyso-sn-glycero-3-phosphocholine in a reaction with 0.5 ml of acetic anhydride and 20 mg of 4-dimethylaminopyridine in 1.25 ml of toluene; the sealed tube was heated at 100° C. for 2 hours and, after evaporation of the solvents with a stream of N2, the products were extracted by the method of Bligh and Dyer as described in Canadian Journal of Biochemical Physiology, Vol. 3, pp. 911-917, 1959. The product, 1-hexadecanoyl-2-acetyl-sn-glycerol-3-phosphocholine, was then treated with phospholipase C to produce 1-hexadecanoyl-2-acetyl-sn-glycerol, which was estimated on the basis of TLC to have a purity >95%.

Provenance & Evidence