HCID 178455898, compound → HCID 178455897, compound

Participants

Reaction Steps

100 mg of Z-Arg-Arg-Pro-Phe-His-Sta-Ile-His-Lys(Boc)-OMe (Example 4) are dissolved in 500 μl of 95% strength TFA, the whole is left to stand for 25 minutes, and precipitation is effected by the addition of 5 ml of diisopropyl ether. The precipitate is filtered off, dried, dissolved in 2 ml of H2O and, in order to be converted into the acetate, filtered slowly through a column (φ=1 cm, length=8 cm) of weakly basic ion exchanger in acetate form. The eluate is concentrated and lyophilised, yielding Z-Arg-Arg-Pro-Phe-His-Sta-Ile-His-Lys-OMe; Rf (D)=0.35; Rf (M)=0.3; Rf (N)=0.3.

Provenance & Evidence