HCID 178455901, compound → HCID 178455900, compound

Participants

Reaction Steps

200 mg of H-Arg-Arg-Pro-Phe-His-Sta-Ile-His-Lys(Boc)-OMe (Example 6) are dissolved in 1 ml of 95% strength TFA, left to stand for 25 minutes and then precipitation is effected by the addition of 10 ml of diisopropyl ether. The precipitate is filtered off, dried, dissolved in 4 ml of H2O and, in order to be converted into the acetate, the solution is slowly filtered through a column (φ=1 cm, length=12 cm) of weakly basic ion exchanger in acetate form. The eluate is concentrated to a small volume and lyophilised, yielding H-Arg-Arg-Pro-Phe-His-Sta-Ile-His-Lys-OMe; Rf (D)=0.15; Rf (M)=0.12; Rf (N)=0.09.

Provenance & Evidence