Ammonia → HCID 178446003, compound

Participants

Reaction Steps

40 mg of H-Pro-Phe-His-Sta-Ala-Sta-OMe are dissolved in 10 ml of saturated methanolic ammonia solution. The solution is left to stand for 48 hours at room temperature, then the solvent is removed in a rotary evaporator and the residue is chromatographed over silica gel in the system chloroform/methanol/concentrated aqueous ammonia solution (40:10:1). The main fractions are combined and concentrated by evaporation. After drying the residue in a high vacuum, H-Pro-Phe-His-Sta-Ala-Sta-NH2 (cf. Example 9) is obtained in the form of a colourless powder; Rf (chloroform/methanol/concentrated ammonia solution [40:10:1])=0.27.

Provenance & Evidence