反应详情

100 mg. of a Escherichia coli acylase that is bound in a polyacrylamide matrix (Boehringer Mannheim) is mixed with an aqueous solution containing 4 mg. of 2-(formylamino)-4-thiazoleacetic acid and an aqueous solution containing 2 mg. of (3S)-3-amino-4-methyl-2-oxo-1-azetidinesulfonic acid, inner salt in a total volume of 2 ml. The pH of the reaction mixture is adjusted to pH 4.5 by the addition of 50 mmolar sodium hydroxide. The reaction mixture is incubated at 30° C. with shaking (60 rpm) for up to 20 hours. The desired product can then be separated from the reaction mixture by thin layer chromatography using a silica gel 60 F254 plate and developing in a solvent consisting of acetonitrile, ethyl acetate, acetic acid, and water (4:4:1:1) followed by elution with water to give (3S)-3-[[[2-(formylamino)-4-thiazolyl]acetyl]amino]-4-methyl-2-oxo-1-azetidinesulfonic acid, zwitterion.

1. additionis mixed with an aqueous solution
2. additioncontaining 4 mg
3. customis incubated at 30° C.
4. customThe desired product can then be separated from the reaction mixture by thin layer chromatography
5. washfollowed by elution with water