Sucrose → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

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As experimental animals, overnight fasted SD rats (Japan SLC. Inc., male, 7-8 weeks of age, 205-272 g) were used. A Test compound was suspended in 0.5% sodium carboxymethylcellulose solution at the concentration of 2 mg/mL. When a homogenous suspension was not obtained in this condition, the test compound was dissolved in ethanol at the concentration of 100 mg/mL of its active form and then 2 mg/mL suspension was obtained by adding this solution to 49 times volumes of 0.5% sodium carboxymethylcellulose solution. Apart of this suspension was diluted with 0.5% sodium carboxymethylcellulose solution and then 0.6 and 0.2 mg/mL suspensions were prepared. After the body weights of rats were measured, the test suspension was orally administered at the dose of 5 mL/kg (1, 3 and 10 mg/kg). For control, just only 0.5% sodium carboxymethylcellulose solution was orally administered at the dose of 5 mL/kg. Immediately after this oral administration, 500 g/L sucrose solution was orally administered at the dose of 5 mL/kg (2.5 g/kg). The oral administration was performed with gastric tube for rat and 2.5 mL syringe. The head count in one group was 3. Collection of urine was performed in metabolic cage after the sucrose administration was finished. The sampling time for collection of urine was 24 hours after the sucrose administration. After collection of urine was finished, the urine volume was recorded and the urinary glucose concentration was measured. The glucose concentration was measured with a kit for laboratory test: Glucose B-Test WAKO (Wako Pure Chemical Industries, Ltd.). The amount of urinary glucose excretion in 24 hours per 200 g of body weight was calculated from urine volume, urinary glucose concentration and body weight. The results are shown in the following Table 4.

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