HuagongsheHuagongshe Chemistry Print Report · For research reference only
Phosphoric Acid → Penicillin G
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Phosphoric Acid | HCID 1004 | — |
| Reactant | Ampicillin | HCID 6249 | — |
| Reactant | 1-Methoxy-2-propanol | HCID 7900 | — |
| Reactant | 6-Aminopenicillanic acid | HCID 11082 | — |
| Reactant | Sodium Hydroxide | HCID 14798 | — |
| Reactant | Sodium Lauryl Sulfate | HCID 3423265 | — |
| Solvent | Acetonitrile | HCID 6342 | — |
| Product | Penicillin G | HCID 5904 | — |
Reaction Steps
Analisys: Concentrations of PGME, 6-APA, ampicillin and PG using HPLC. Column Waters C18: Nova-Pak, 60 Å, diameter 4 μm, 3.9×150 mm. Mobile phase: 35% acetonitrile, 2‰ SDS (sodium dodecyl sulfate), 10 mM H3PO4, 5 mM K2H2PO4, pH 4.6 and 25° C., 225 nm, flow 1 mL/min. Synthesis experiments: 25° C., pH 6.5 controlled by automatic addition of NaOH. Jacketed glass vortex flow reactor, internal cylinder of stainless steel 316. Reaction volume, 50 mL. Reactor internal diameter, 36 mm. Rotary cylinder diameter, 10 mm (radius ratio 0.28). Volume of biocatalyst, 20 mL. Enzymatic load, 10 IU/(mL of reactor).
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US07648831B2 |
| Source dataset | uspto-grants-2010_01 |
| ORD ID | ord-21741b5378c245d180a653c4cf89bc60 |
| Source note | uspto-grants-2010_01 |