反应详情

React 1-(5-tert-butyl-2-p-tolyl-2H-pyrazol-3-yl)-3-(6-piperazin-1-yl-pyridin-3-yl)-urea (0.20 g, 0.46 mmol) with 3-methyl-pentanoic acid (0.059 g, 0.51 mmol), 1-hydroxybenzotriazole hydrate (0.06 g, 0.46 mmol) and polymer supported carbodiimide (0.83 g, 1.0 mmol), suspended in a mixture CH2Cl2/DMF (18/1, mL). Stir the mixture at room temperature overnight and then filter and wash the resin with CH2Cl2. Concentrate and purify the residue with a SCX cartridge eluting with NH4OH/CH3OH 2N to yield 135 mg (55%) of the title compound as a pale pink solid. MS(ES+): m/z=532 [M+H].

1. filtrationfilter
2. washwash the resin with CH2Cl2
3. concentrationConcentrate
4. custompurify the residue with a SCX cartridge
5. washeluting with NH4OH/CH3OH 2N