Pyridine → 3-Methoxy-7beta-phenylacetylamino-3-cephem-4-carboxylic acid diphenylmethyl ester

HRID 1995804Notes

Reaction equation

HRID 1995804 reaction equation
[C:1]1([CH:7]([O:14][C:15]([C:17]2[N:22]3[C:23](=[O:26])[C@@H:24]([NH2:25])[C@H:21]3[S:20][CH2:19][C:18]=2[O:27][CH3:28])=[O:16])[C:8]2[CH:13]=[CH:12][CH:11]=[CH:10][CH:9]=2)[CH:6]=[CH:5][CH:4]=[CH:3][CH:2]=1.N1C=CC=CC=1.[C:35]1([CH2:41][C:42](Cl)=[O:43])[CH:40]=[CH:39][CH:38]=[CH:37][CH:36]=1>C(Cl)Cl>[C:1]1([CH:7]([O:14][C:15]([C:17]2[N:22]3[C:23](=[O:26])[C@@H:24]([NH:25][C:42](=[O:43])[CH2:41][C:35]4[CH:40]=[CH:39][CH:38]=[CH:37][CH:36]=4)[C@H:21]3[S:20][CH2:19][C:18]=2[O:27][CH3:28])=[O:16])[C:8]2[CH:9]=[CH:10][CH:11]=[CH:12][CH:13]=2)[CH:6]=[CH:5][CH:4]=[CH:3][CH:2]=1

Participants

5

Reactant 3

HCID 1049PyridineHCID 7679Phenylacetyl chlorideHCID 441511537beta-Amino-3-methoxy-3-cephem-4-carboxylic acid diphenylmethyl ester

Product 1

HCID 128054463-Methoxy-7beta-phenylacetylamino-3-cephem-4-carboxylic acid diphenylmethyl ester

Reagents, catalysts & solvents 1

HCID 6344DichloromethaneSolvent

Procedure

A suspension of 0.250 g of 7β-amino-3 -methoxy-3-cephem-4-carboxylic acid diphenylmethyl ester in 25 ml of methylene chloride is treated with 1 ml of pyridine and 0.5 ml of phenylacetic acid chloride at 0° C. under a nitrogen atmosphere and stirred for 30 minutes at this temperature. The reaction mixture is evaporated under reduced pressure; the residue is stirred for 10 minutes with 20 ml of a 1:1 mixture of dioxane and water and the mixture is diluted with methylene chloride. The aqueous phase is separated off and extracted with methylene chloride. The combined organic phases are washed with a saturated aqueous sodium bicarbonate solution and a saturated aqueous sodium chloride solution, dried over sodium sulphate and evaporated under reduced pressure. The crude product is purified by means of preparative thin layer chromatography, using a 1:1 mixture of toluene and ethyl acetate as the solvent. The zone visible under ultraviolet light of λ=254 mμ (Rf~0.35) is eluted with a 4:1 mixture of acetone and methanol and yields 3-methoxy-7β-phenylacetylamino-3-cephem-4-carboxylic acid diphenylmethyl ester, which is identical with the product obtainable according to the process of Example 1.

Workup

  1. customThe reaction mixture is evaporated under reduced pressure
  2. stirringthe residue is stirred for 10 minutes with 20 ml of a 1:1 mixture of dioxane and water
  3. additionthe mixture is diluted with methylene chloride
  4. customThe aqueous phase is separated off
  5. extractionextracted with methylene chloride
  6. washThe combined organic phases are washed with a saturated aqueous sodium bicarbonate solution
  7. dry with materiala saturated aqueous sodium chloride solution, dried over sodium sulphate
  8. customevaporated under reduced pressure
  9. customThe crude product is purified by means of preparative thin layer chromatography
  10. additiona 1:1 mixture of toluene and ethyl acetate as the solvent
  11. washThe zone visible under ultraviolet light of λ=254 mμ (Rf~0.35) is eluted with a 4:1 mixture of acetone and methanol

Sources & evidence

ORD record
ord-1c76b9b3796648d08faeb3a3ec245f0e
Source dataset
uspto-grants-1983_09
Patent number
US04405778

Reaction notes

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