反应详情

温度

60 °C

A mixture of the product of Example 31, step B (18 mg, 0.04 mmol) and 33% HBr in acetic acid (10 mL) was heated at 60° C. for 4 days. The reaction mixture was concentrated in vacuo and the resulting residue neutralized with saturated sodium bicarbonate solution before extracting with dichloromethane. The organic layer was dried over sodium sulfate, filtered and concentrated in vacuo. The crude material was purified by semi-preparative HPLC and the free base treated with 1.25 M HCl methanol solution (0.5 mL) to give 2-(4-hydroxyphenyl)sulfonyl-5-methyl-5,6,7,8,9,10-hexahydro-7,10-epiminocyclohepta[b]indole hydrochloride (2 mg, 12%, AUC HPLC 97.2%) as an off-white solid: mp 210-212° C.; 1H NMR (CD3OD, 300 MHz) δ 8.18-8.23 (m, 1H), 7.73-7.81 (m, 2H), 7.69 (dd, J=9.0 Hz, 1.8 Hz, 1H), 7.55 (d, J=8.7 Hz, 1H), 6.82-6.90 (m, 2H), 5.25-5.36 (m, 1H), 4.53 (br s, 1H), 3.71 (s, 3H), 3.40-3.55 (m, 1H), 3.46-3.55 (m, 1H), 3.01-3.11 (m, 1H), 2.23-2.51 (m, 2H), 1.92-2.11 (m, 1H); ESI MS m/z 369 [M+H]+.

1. concentrationThe reaction mixture was concentrated in vacuo
2. extractionbefore extracting with dichloromethane
3. dry with materialThe organic layer was dried over sodium sulfate
4. filtrationfiltered
5. concentrationconcentrated in vacuo
6. customThe crude material was purified by semi-preparative HPLC
7. additionthe free base treated with 1.25 M HCl methanol solution (0.5 mL)