Ammonia → HCID 86665779, compound

Participants

Reaction Steps

Crude 1-(2,3-Dihydroxy-4-hydroxymethyl-cyclopentyl)-3-methoxycarbonyl-pyridinium-methansulfonate (118.3 g, 173.7 mmol) was dissolved in 100.0 ml methanol. After the addition of methanolic ammonia (7M, 350.0 ml, 2.45 mol) the reaction mixture was stirred for 2.5 hrs. After removing the solvent under reduced pressure, a red-brown oil was obtained that was further dried for 3 hrs. (40° C., 10 mbar). This crude product may be pre-purified with activated charcoal and may be used directly for the chemical synthesis of cNAD (WO2007/012494) or in the enzymatic synthesis of cNAD as described herein.

Provenance & Evidence