反应详情

{2-[3-(3-methyl-butyl)-3-(trans-4-methyl-cyclohexyl)-ureido]-thiazole-5-sulfonylamino}-acetic acid ethyl ester was dissolved in MeOH and treated with 15 equivalents of 1N NaOH for 2 days at room temperature. MeOH was removed by evaporation. Addition of 1N HCl to pH<1 caused precipitation. The precipitate was isolated by filtration, washed with water and dried to give {2-[3-(3-methyl-butyl)-3-(4-methyl-cyclohexyl)-ureido]-thiazole-5-sulfonylamino}-acetic acid as crystals.

1. customMeOH was removed by evaporation
2. additionAddition of 1N HCl to pH<1
3. customprecipitation
4. customThe precipitate was isolated by filtration
5. washwashed with water
6. customdried