HuagongsheHuagongshe Chemistry Print Report · For research reference only
NADP nicotinamide-adenine-dinucleotide phosphate → Nadh
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | NADP nicotinamide-adenine-dinucleotide phosphate | HCID 5886 | — |
| Reactant | Shikimic Acid | HCID 8742 | — |
| Reactant | Tromethamine hydrochloride | HCID 93573 | — |
| Product | Nadh | HCID 439153 | — |
Reaction Steps
Each of these crude enzyme solutions was added to a mixture of 0.1M Tris-HCl buffer (pH 7.4), 0.8 mM NADP and 4 mM shikimic acid, and shikimate dehydrogenase activity was determined by measuring the change in optical density at 300 mμ resulting from the conversion of NADH.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US05017481 |
| Source dataset | uspto-grants-1991_05 |
| ORD ID | ord-af27e2d7ec684b7f9da7414b8be957d3 |
| Source note | uspto-grants-1991_05 |