Trifluoroacetic acid → HCID 10044232, compound

Participants

Reaction Steps

6-Cyclopropylamino-9-(4-methoxycarbonylphenyl)-2-trifluoromethylpurine (20 mg, 0.053 mmol) was treated with 0.53 mmol of 1-2 M KOH in methanol (containing 5% water) and stirred for 14 hours at room temperature. The reaction mixture was acidified with trifluoroacetic acid (30% in dichloromethane) to pH=2 and concentrated under vacuum. The residue was extracted with 20% methanol in dichloromethane. The extraction was loaded on a silica gel column and eluted with 20% methanol in dichloromethane to give 9-(4-carboxyphenyl)-6-cyclopropylamino-2-trifluoromethylpurine (14 mg, 70% yield). 1H NMR (300 MHz, DMSO) δ 8.85 (s, 1H), 8.70 (s, 1H), 8.15 (d, J=8.4 Hz, 2H), 8.01 (d, J=8.4 Hz, 2H), 3.07 (b, 1H), 0.78 (b, 2H), 0.68 (b, 2H). [ESMS m/z (363.9 (M+1)+)]

Provenance & Evidence