L-Glutamine → L-Theanine

HRID 2056236Notes

Reaction equation

HRID 2056236 reaction equation
[CH2:1](N)[CH3:2].[NH2:4][C@H:5]([C:11]([OH:13])=[O:12])[CH2:6][CH2:7][C:8](=[O:10])[NH2:9]>>[NH2:4][C@H:5]([C:11]([OH:13])=[O:12])[CH2:6][CH2:7][C:8]([NH:9][CH2:1][CH3:2])=[O:10]

Participants

3

Reactant 2

HCID 5961L-GlutamineHCID 6341Ethylamine

Product 1

HCID 439378L-Theanine

Procedure

Ten gram of the prepared cells were used for enzymatic reaction in 0.3M glutamine, and 0.9M ethylamine at 30° C. and pH 10. Forty grams of theanine were obtained from 1 liter after incubating at 30° C. for 24 hours. Theanine was extracted from the reaction mixture by eliminating cells first and fractionated by passing through Dowex50×8 and Dowex 1×2 columns serially. The theanine fraction was crystallized and washed with ethanol.

Workup

  1. customat 30° C.

Sources & evidence

ORD record
ord-4b301f63006d4cac8a3f35e2616e9dac
Source dataset
uspto-grants-2008_02
Patent number
US07335497B2

Reaction notes

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