Dimethylformamide → HCID 131711814, compound

Participants

Reaction Conditions

Reaction Steps

3.4 g (16.4 mmoles) of DCCI are added at 0° C. to a solution of 22.8 g (14 mmoles) of H-Tyr(But)-Gln-Leu-Glu(OBut)-Asn-Tyr(But)-Cys(Trt)-Asn-OBut, 14.1 g (15.4 mmoles) of Ddz-Ile-Cys(Trt)-Ser-Leu-OH.1 H2O, 2.08 g (15.4 mmoles) of HOBt and 1.8 ml (14 mmoles) of N-ethylmorpholine in 70 ml of dimethylformamide. The mixture is stirred for 1 hour at 0° C. and 3 hours at room temperature and is then left to stand overnight at room temperature, and the precipitate is filtered off. The filtrate is concentrated in vacuo and the residue is successively triturated with NaHCO3 solution, citrate buffer (pH 3), NaHCO3 solution and water and is filtered off in each case. It is dried over P2O5.

Provenance & Evidence