Dimethyl Sulfoxide → HCID 86628350, compound

Participants

Reaction Steps

In-bred DIO prone rats were obtained from Charles Rivers Labs. These rats were developed from a line of Crl:CD®(SD)BR rats that are prone to become obese on a diet relatively high in fat and energy (Levin, 1997). The rats were housed individually in shoebox cages at 22° C. in a 12/12-hour light dark cycle. Rats were maintained ad-libitum on a moderately high fat diet (32% kcal from fat; Research Diets D1226B) for 6 weeks prior to drug treatment. At the end of the fattening period they typically have a mean body weight of 500 g. Rats were then divided into treatment groups and implanted with two subcutaneous mini-pumps (Durect Corp). One pump contained either vehicle (50% DMSO in water) or amylin (100 μg/kg/day) while the other pump contained either sterile water or sibutramine (3 mg/kg/day).

Provenance & Evidence