反应详情

温度

50 °C

A solution of N-cyclopropyl-4-methyl-3-[3-{[1-methyl-1-(2-{[(4S)-2-oxo-1,3-oxazolidin-4-yl]methoxy}phenyl)ethyl]amino}-2-oxopyrazin-1(2H)-yl]benzamide (Example 305a) (0.6 g) in water (10 mL) and MeOH (10 mL) was treated with potassium hydroxide (0.130 g) under nitrogen. The resulting mixture was stirred at 50° C. for 16 h. The reaction mixture was diluted with water (200 mL), and extracted with ethyl acetate (250 mL). The organic phase was dried (MgSO4), filtered and evaporated. Purification by preparative HPLC (Phenomenex Gemini column, eluting with a gradient of acetonitrile in 0.2% (v/v) aqueous ammonia) gave the title compound as a solid (0.105 g).

1. extractionextracted with ethyl acetate (250 mL)
2. dry with materialThe organic phase was dried (MgSO4)
3. filtrationfiltered
4. customevaporated
5. customPurification by preparative HPLC (Phenomenex Gemini column
6. washeluting with a gradient of acetonitrile in 0.2% (v/v) aqueous ammonia)