Citric Acid → HCID 86735086, compound

Participants

Reaction Steps

10.66 g of amphotericin B (938γ/mg of activity-equivalent to 10 g of chemical activity) suspended in 2.5 liters anhydrous methanol containing 11.1 ml of 5% CaCl2 in methanol (equivalent to 200 mg Ca ion) is solubilized by means of 1.75 ml of 5.8 N HCl (pH 3.9). To the resulting solution is added 10.9 g of reagent grade citric acid. H2O (equivalent to 10 g anhydrous citric acid) to form a mixture of pH 2. The pH of the mixture is adjusted to about 7 by the addition of 7.9 ml concentrated NH4OH. The mixture is then heated to 45°-50° C. and slurried for 1 hour and thereafter is cooled to room temperature, filtered and the wet cake dried overnight under vacuum at 45°-50° C. to yield 10 g of complex.

Provenance & Evidence