L-(-)-Sorbose → Sorbosone, L-

Participants

Reaction Steps

The reaction mixture containing 100 ml of cell free extract of UV-10, FERM BP-1267 (total enzyme activity, 123 units), as prepared by the manner as described in steps (1) to (2) of Example 1, 50 ml of 0.5M potassium phosphate buffer (PH 6.0), 50 ml of 1M L-sorbose solution, 10 ml of 0.2M phenazine methosulfate solution and 290 ml of water was incubated at 30° C. with gentle shaking. As a result, L-sorbosone was formed with the rate of 142 mg/hr.

Provenance & Evidence