Phosphate → (-)-Carnitine

Cells were collected by centrifugation from 300 ml of the culture broth obtained by cultivating the strain CA28-50A in the same medium as used in Example 9 containing 0.5% DL-carnitinamide and ground in a 50 mM phosphate buffer solution (pH 7.0) together with quartz sand followed by centrifugation to obtain 30 ml of a cell-free extract. Living cells and a cell-free extract were each added to a reaction solution containing 0.2% DL-carnitinamide in the same concentration as that in the growth culture medium in terms of the original cell amount and the resulting mixtures were allowed to stand for the reaction at 26° C. Analytical data on the reaction time and the amount of L-carnitine formed are shown in Table 7 below.

1. customCells were collected by centrifugation from 300 ml of the culture broth
2. customobtained
3. customto obtain 30 ml of a cell-free extract
4. extractionLiving cells and a cell-free extract
5. additioneach added to a reaction solution
6. additioncontaining 0.2% DL-carnitinamide
7. concentrationin the same concentration as that in the growth culture medium in terms of the original cell amount
8. customto stand for the reaction at 26° C

ORD record

ord-025bfeaa7f4443c0828603197cf83f0b

Source dataset

uspto-grants-1990_04

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US04918012