HuagongsheHuagongshe Chemistry Print Report · For research reference only
Phenylmethylsulfonyl Fluoride → Kaurenoic Acid
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Phenylmethylsulfonyl Fluoride | HCID 4784 | — |
| Reactant | Ethylenediaminetetraacetic Acid | HCID 6049 | — |
| Reactant | Tris(hydroxymethyl)aminomethane | HCID 6503 | — |
| Reactant | D-1,4-Dithiothreitol | HCID 446094 | — |
| Reactant | Flavin adenine dinucleotide | HCID 643975 | — |
| Reactant | [[(2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2S,3R,4S,5S)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate | HCID 71717786 | — |
| Solvent | ent-Kaur-16-en-19-oic acid | HCID 3034087 | — |
| Product | Kaurenoic Acid | HCID 73062 | — |
Reaction Steps
For each gene 5 ml of expression culture was pelleted and resuspended in 1 ml of kaurenoic acid assay buffer (100 mM Tris (pH 7.5), 1 mM DTT (dithiothreotol), 0.5 mM NADPH (nicotinamide adenine dinucleotide phosphate), 0.5 mM FAD (flavin-adenine dinucleotide), 0.05 mg/ml kaurenoic acid, 0.05× Complete EDTA-free protease inhibitor cocktail (Roche), 0.4 μM PMSF (phenylmethylsulphonylfluoride)) and incubated at 30° C. for 5 h at 850 rpm in an Eppendorf Thermomixer (Westbury, N.Y.).
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US07927851B2 |
| Source dataset | uspto-grants-2011_04 |
| ORD ID | ord-3ba5b74f9231459faee8c3ed0967a775 |
| Source note | uspto-grants-2011_04 |