Dimethyl Sulfoxide → HCID 101591928, compound

Participants

Reaction Steps

0.25 g of milbemycin A4 and 5 ml of DMSO are added to a 1-day-old culture of S. violascens in 200 ml of medium 3 and the whole is shaken at 250 rpm. at 28° C. After 3 days, a further 50 ml of medium 3 is added. The course of the reaction is monitored by HPLC (column Kontron LiSi 60, 25 cm/0.5 cm, eluant hexane/dimethoxyethane 3:1, 1 ml/min., UV detector 220 nm). After shaking for 7 days and a 91% conversion rate, 92% 13β-hydroxymilbemycin A4 and 8% 14,15-epoxymilbemycin A4 are obtained.

Provenance & Evidence