Water → Uric Acid
Reaction equation
[NH:1]1[C:10](=[O:11])[C:9]2[NH:8][CH:7]=[N:6][C:5]=2[NH:4][C:2]1=[O:3].[OH2:12]>>[NH:1]1[C:10](=[O:11])[C:9]2[NH:8][C:7](=[O:12])[NH:6][C:5]=2[NH:4][C:2]1=[O:3]Participants
3
Reactant 2
Product 1
Conditions
- Temperature
- 37 °C
Procedure
50 mM tris-hydrochloric acid buffer (2.9 ml) and 0.1 ml of a 10 mM aqueous solution of xanthine are mixed, and this mixture is pre-heated at 37° C. After 0.01 ml of enzyme solution is added and gently mixed, the increase of absorbance at 293 nm per minute is determined using a spectrophotometer thermostated at 37° C. with distilled water as the control. The xanthine oxidase activity is expressed on the assumption that the amount of enzyme which produces 1 μmol of uric acid per minute under the conditions described above is taken as 1 unit (U).
Workup
- additiongently mixed
- temperaturethe increase of absorbance at 293 nm per minute
- customat 37° C.
Sources & evidence
- ORD record
- ord-bd3d049c7b774c7685afe1087fb1948a
- Source dataset
- uspto-grants-1993_02
- Patent number
- US05185257
Reaction notes
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