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A culture of Hormodendrum cladosporioides NRRL 8132 is grown initially for 14 days on potato dextrose agar slants. The spores and mycelia from a single slant are used to inoculate a 500 ml. seed flask containing 100 ml. of cottonseed meal medium. After further incubation for 7 days at 23 - 25° C. on a rotary shaker at 190 rpm, 25 ml. of the growth is used to inoculate 100 ml. of cottonseed meal medium in a 500 ml. flask. This flask is then incubated at 23 - 25° C. for 24 - 48 hours on the shaker until good growth is evident. A solution of 0.025 part of 8-chloro-10,11-dihydrodibenz[b,f][1,4]oxazepine in 0.79 part acetone is added and incubation continued for an additional 48 hours. The culture broth is extracted three times with portions of methylene chloride. The methylene chloride extracts are combined, dried over anhydrous sodium sulfate, and stripped to dryness in vacuo. The residue is dissolved in ethyl acetate and chromatographed on silica gel using a 3:97 ethyl acetate-methylene chloride mixture as eluant. Recrystallization of the crude product affords 8-chlorodibenz[b,f][1,4]oxazepine melting at 73 - 73.5° C. and represented by the following structural formula. ##STR4##

1. additionseed flask containing 100 ml
2. waitThis flask is then incubated at 23 - 25° C. for 24 - 48 hours on the shaker until good growth
3. waitincubation continued for an additional 48 hours
4. extractionThe culture broth is extracted three times with portions of methylene chloride
5. dry with materialdried over anhydrous sodium sulfate
6. dissolutionThe residue is dissolved in ethyl acetate
7. customchromatographed on silica gel using a 3:97 ethyl acetate-methylene chloride mixture as eluant
8. customRecrystallization of the crude product