反应详情

A greased VDX plate and circle siliconized glass cover slides were used. 500 μL of a particular reservoir solution was prepared by admixing ammonium sulfate stock solution, Bis-Tris propane stock solution and Milli Q water in each well. In this example, ammonium sulfate molarity was around 0.5 M, 1 M, 1.5 M or 2 M. The Bis-Tris Propane molarity was 0.1 M throughout, and the Bis-Tris Propane buffer pH was around 5.5, 6.0, 6.5, 7.0, 7.5 or 8.0. The resulting 24 conditions were assessed with all of the three protein concentrations as described above, and with storage at ambient temperature or storage at around 27° C., respectively. Around 1 μL of protein solution was admixed with around 1 μL of a particular reservoir solution on a circle siliconized glass cover slide, and the well was sealed with the inverted slide, generating a hanging drop experiment. The plates were stored at ambient temperature. Microscopy of the drops was performed after three days. The conditions were classified into clear drops, drops containing random precipitation, drops containing crystals and drops containing mixtures of precipitated species and crystals.

1. concentrationof the three protein concentrations
2. customat ambient temperature
3. customat around 27° C.
4. customthe well was sealed with the inverted slide
5. customwere stored at ambient temperature
6. additioncontaining random
7. customprecipitation, drops
8. additioncontaining crystals and drops
9. additioncontaining mixtures of precipitated species and crystals