Acetate → HCID 86638818, compound

HRID 2090135Notes

Reaction equation

HRID 2090135 reaction equation
[C:1]([O-:4])(=[O:3])[CH3:2].[P:5]([O-:9])([OH:8])([OH:7])=[O:6].[Na+:10].[P:11]([O-:15])([OH:14])([OH:13])=[O:12].[K+:16]>O>[P:5]([O-:9])([OH:8])([OH:7])=[O:6].[K+:16].[Na+:10].[P:11]([O-:15])([OH:14])([OH:13])=[O:12].[C:1]([O-:4])(=[O:3])[CH3:2].[Na+:10]

Participants

5

Reactant 3

HCID 175Acetate3×HCID 516951Monobasic potassium phosphate3×HCID 23672064Monosodium phosphate3×

Product 1

HCID 86638818HCID 86638818, compound

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

Batch crystallization was performed by admixing around 400 μL of each protein solution with an equal amount of crystallization solution in a 1.5 mL Eppendorff reaction tube. 400 μL of a particular crystallization solution was prepared by admixing acetate buffer, sodium dihydrogen phosphate stock solution, potassium dihydrogen phosphate stock solution and Milli Q water. In this example, the acetate buffer molarity was 0.1 M, and the acetate buffer pH was around 4.1. The following combination of sodium dihydrogen phosphate and potassium dihydrogen phosphate was used: around 0.9 M sodium dihydrogen phosphate and around 0.9 M potassium dihydrogen phosphate. The reaction tubes were stored at ambient temperature. Microscopy of 1 μL aliquots was performed after 11 days.

Workup

  1. customBatch crystallization
  2. customwith an equal amount of crystallization solution in a 1.5 mL
  3. customEppendorff reaction tube
  4. customThe reaction tubes
  5. customwere stored at ambient temperature

Sources & evidence

ORD record
ord-b09a058af0b042729bff9147ac1eab80
Source dataset
uspto-grants-2013_05
Patent number
US08436149B2

Reaction notes

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