Acetate → HCID 86638819, compound

HRID 2090137Notes

Reaction equation

HRID 2090137 reaction equation
[C:1]([O-:4])(=[O:3])[CH3:2].[P:5]([O-:9])([OH:8])([OH:7])=[O:6].[Na+:10]>O>[P:5]([O-:9])([OH:8])([OH:7])=[O:6].[Na+:10].[C:1]([O-:4])(=[O:3])[CH3:2].[Na+:10]

Participants

4

Reactant 2

HCID 175Acetate3×HCID 23672064Monosodium phosphate2×

Product 1

HCID 86638819HCID 86638819, compound

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

Batch crystallization was performed by admixing around 1 mL of the protein solution with an equal amount of crystallization solution in a 2 mL Eppendorff reaction tube. 1 mL of a particular crystallization solution was prepared by admixing acetate buffer, sodium dihydrogen phosphate stock solution and Milli Q water. In this example, the acetate buffer molarity was 0.1 M, and the acetate buffer pH was around 4.1. Sodium dihydrogen phosphate molarity was around 4.0 M, 4.2 M or 4.4 M. The reaction tubes were stored at ambient temperature. Microscopy of 1 μL aliquots was performed multiple times during the following week.

Workup

  1. customBatch crystallization
  2. customwith an equal amount of crystallization solution in a 2 mL
  3. customEppendorff reaction tube
  4. customThe reaction tubes
  5. customwere stored at ambient temperature

Sources & evidence

ORD record
ord-6c3a358823e34495bac9a8ede3760501
Source dataset
uspto-grants-2013_05
Patent number
US08436149B2

Reaction notes

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