Hydrochloric Acid → HCID 11685952, compound

Participants

Reaction Steps

0.28 g of tert-butyl (2S,4R)-2-({[(1R)-1-(4-chlorobenzyl)-2-(3-{cyclohexyl[(diethylamino)carbonyl]-amino}-8-azabicyclo[3.2.1]oct-8-yl)-2-oxoethyl]amino}-methyl)-4-hydroxypyrrolidine-1-carboxylate is dissolved in 5 ml of diethyl ether, and then 2.7 ml of 2N hydrochloric acid in diethyl ether are added. Trituration, rinsing with diethyl ether, and filter-drying of the precipitate obtained are carried out, which precipitate is chromatographed on silica gel, elution being carried out with a 90/10/1 mixture of dichloromethane, methanol and aqueous ammonia. 0.18 g of N-[8-(4-chloro-N-{[(2S,4R)-4-hydroxypyrrolidin-2-yl]methyl}-D-phenylalanyl)-8-azabicyclo[3.2.1]oct-3-yl]-N-cyclohexyl-N′,N′-diethylurea is obtained.

Provenance & Evidence