Potassium Phosphate, Tribasic → Monatin

HRID 2100245Notes

Reaction equation

P([O-])([O-])([O-])=O.[K+].[K+].[K+].[NH2:9][C@H:10]([C:16]([O-:18])=[O:17])[CH2:11]CC([O-])=O.[NH:19]1[C:27]2[C:22](=[CH:23][CH:24]=[CH:25][CH:26]=2)[CH:21]=[C:20]1CC(=O)C([O-])=O.[Mg+2].[Cl-].[Cl-].[C:37]([O-:42])(=[O:41])[C:38]([CH3:40])=[O:39]>>[CH:24]1[CH:23]=[C:22]2[C:21]([CH2:40][C@@:38]([OH:39])([C:37]([OH:42])=[O:41])[CH2:11][C@H:10]([NH2:9])[C:16]([OH:18])=[O:17])=[CH:20][NH:19][C:27]2=[CH:26][CH:25]=1

Participants

Reactant 5

HCID 62657Potassium Phosphate, Tribasic

HCID 107735Pyruvate

HCID 5360315Magnesium Chloride

HCID 5460544L-glutamate(2-)

HCID 129900254HCID 129900254, compound

Product 1

HCID 9860847Monatin

Procedure

Similar experiments were conducted using Biocatalytics Aminotransferase buffer, which contained 100 mM potassium phosphate pH 7.5, 0.1 mM PLP, and 100 mM glutamate. Solid indole-pyruvate and D-aminotransferase were added as above. ProA aldolase (50 μg), MgCl2, and 50 mM pyruvate were added from stock solutions. The assays were treated as above, although no pH adjustment was required in this case. A negative control was done with just the BioCatalytics supplied enzyme and buffer, which did not produce any monatin. The experimental results are shown in Table 9.

Workup

additionThe assays were treated as above, although no pH adjustment

Sources & evidence

ORD record: ord-ca44e92050f142ab93ecc295455f5589
Source dataset: uspto-grants-2009_08
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US07572607B2

Reaction notes

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